5-TAMRA Picolyl Azide|5-TAMRA Picolyl N3
5-tamra吡啶甲基叠氮是一种先进的荧光探针，采用铜螯合基序提高铜的有效浓度（我）在反应位点。螯合作用的吡啶甲基叠氮基团在反应部位提高铜的有效浓度是如此之大，导致高达40倍的信号强度的增加。此外，代替传统的5-tamra叠氮化物5-tamra吡啶甲基叠氮的使用允许至少十倍减少铜催化剂的浓度不牺牲标记效率。
虽然5的混合异构体（6）- TAMRA是*，经常使用的红色荧光染料染色的蛋白质，肽和核苷酸标记TAMRA混合异构体可能麻烦由于显著的信号在HPLC纯化展宽。肽和核苷酸标记与一个单一的异构体发生通常会得到更好的分辨率在HPLC纯化，往往在接合过程中需要。
总之，一个甲基吡啶基团为叠氮探针的引入在炔烃的检测灵敏度大幅提高。这对于探测低丰度目标或显著提高信号强度是特别有价值的。
 
Abs/Em Maxima

545/576
Extinction coefficient

91,000
Flow cytometry laser line

532 or 555 nm
Microscopy laser line

532 or 555 nm
Spectrally similar dyes

Alexa Fluor® 546, Atto™ 543, CF™ 555 Dye, DyLight™549
Molecular weight

808.89
CAS

N/A
Solubility

DMSO, DMF, MeOH
Purity

>97% (HPLC)
Appearance

Red solid
Storage conditions

-20C
Shipping conditions

Ambient temperature
5-TAMRA Picolyl Azide|5-TAMRA Picolyl N3
叠氮化合物(Azides)
Azidobutyric acid NHS ester
Azido dyes, available as solids, and as ready-to-use 10 mM DMSO solutions
Water-soluble Cy3 azide
Water-soluble Cy5 azide
Cy3 azide
Cy3.5 azide
Cy5 azide
Cy5.5 azide
Cy7 azide
Cy7.5 azide
5-FAM azide
6-FAM azide
5-JOE azide
5-R110 azide
6-R110 azide
6-ROX azide
5-TAMRA azide
炔烃类染料(Alkynes)
Alkyne phosphoramidite, 5'-terminal
Alkyne amidite, hydroxyprolinol
Pentynoic acid STP ester
荧光激活染料(Catalysts and activators)
Ascorbic acid
Copper(II)-TBTA plex, 10 mM in 55% a. DMSO
SYBR Green I
Gel staining solution, 10000x
For PCR, 100x solution
改良三磷酸盐(Modified triphosphates)
Ami1-ddUTP
Ami1-dUTP
脂类活性染料(Dye activated esters)
Cy3 NHS ester (water soluble)
Cy5 NHS ester (water soluble)
Cy3 NHS ester
Cy3.5 NHS ester
Cy5 NHS ester
Cy5.5 NHS ester
Cy7 NHS ester
Cy7.5 NHS ester
ROX NHS ester, pure 6- isomer
羰基活性染料(Dye hydrazides)
Cy5 hydrazide
巯基反应性染料(Dye maleimides)
Cy5 maleimide
Cy5.5 maleimide
2D DIGE荧光染料试剂盒(2D DIGE dye kits)
Phosporamidites
Alkyne phosphoramidite, 5'-terminal
Alkyne amidite, hydroxyprolinol
DMS(O)MT aminolink amidite
Long trebler amidite
Pyrene dU amidite