BDP FL tetrazine,氟化硼二吡咯-四嗪
Inverse electron demand Diels-Alder reaction (ied-DA) with tetrazines is a promising tool for the conjugation of biomolecules. The reaction takes place between tetrazine as an electron acceptor heterodiene, and a strained dienophile, such as trans-cyclooctene, cyclopropene, or some cyclooctynes.
Methyltetrazine provides greater stability in buffers than unsubstituted tetrazine. The rate of its reaction with cycloalkenes still beats almost all other conjugation reaction rates by a factor of magnitudes.
BDP FL is a bright dye for fluorescein (FAM) channel. Using BDP FL tetrazine, the dye can be conjugated with molecules bearing strained olefins.
General properties
Appearance:

red crystalline solid
Mass spec M+ increment:

447.2
Molecular weight:

475.3
Molecular formula:

C24H24N7BF2O
Solubility:

good in DCM, DMSO, DMF
Quality control:

NMR 1H, HPLC-MS (95%)
Storage conditions:

Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Spectral properties
Excitation maximum, nm:

503
ε, L⋅mol−1⋅cm−1:

80000
Emission maximum, nm:

509
Fluorescence quantum yield:

0.97
CF260:

0.015
CF280:

0.027
BDP FL tetrazine,氟化硼二吡咯-四嗪
马来酰亚胺 Dye maleimides
Pyrene maleimide
BDP FL maleimide
Cyanine3 maleimide
Cyanine5 maleimide
Cyanine5.5 maleimide
Cyanine7 maleimide
Cyanine7.5 maleimide
Sulfo-Cyanine3 maleimide
Sulfo-Cyanine5 maleimide
伯胺 Primary amine
BDP FL amine
Cyanine3 amine
Cyanine5 amine
Cyanine5.5 amine
Cyanine7 amine
Cyanine7.5 amine
Sulfo-Cyanine5 amine
Sulfo-Cyanine7 amine
酰肼 Dye hydrazides
Pyrene hydrazide
BDP FL hydrazide
Cyanine3 hydrazide
Cyanine5 Boc-hydrazide
Cyanine5 hydrazide
Cyanine5.5 hydrazide
Cyanine7 hydrazide
Cyanine7.5 hydrazide