Cyanine5.5 hydrazide|Cy5.5 hydrazide
Cyanine5.5 hydrazide is a carbonyl-reactive near infrared dye. Hydrazides quickly react with carbonyl compounds, such as aldehydes and ketones, and form stable hydrazones. This reaction is useful for the preparation of labeled conjugates.
Carbonyl groups in biomolecules can form spontaneously after oxidative stress or deamination of proteins. Alternatively, aldehyde groups can be specifically generated by periodate oxidation of sugar residues of glycoproteins, for example antibodies. Antibodies can be oxidized by sodium periodate and labeled with Cyanine5.5 hydrazide to form Cyanine5.5 labeled antibodies. Since sugar residues in antibodies are located far from variable, epitope-binding site, this method is particularly good for the labeling of antibodies.
Cyanine5.5 is a near infrared fluorophore, an analog of Cy5.5®. It is particularly suitable for the applications which require low fluorescence background. The absorption and emission wavelength of the dye exhibit significant tissue penetration, so Cyanine5.5 is suitable for in vivo NIR imaging applications.
Cyanine5.5 hydrazide|Cy5.5 hydrazide
General properties：
Appearance:
dark blue powder
Molecular weight:
669.73
Molecular formula:
C40H46Cl2N4O
Solubility:
moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)
Storage conditions:
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
Spectral properties：
Excitation maximum, nm:
684
ε, L⋅mol−1⋅cm−1:
209000
Emission maximum, nm:
710
Fluorescence quantum yield:
0.2
CF260:
0.07
CF280:
0.03
酰肼染料 DYE HYDRAZIDES
Cy3酰肼Cyanine3 hydrazide
Cy5酰肼 Cyanine5 hydrazide
Cy5 Boc-酰肼 Cyanine5 Boc-hydrazide
Cy5.5酰肼 Cyanine5.5 hydrazide
酰肼 Dye hydrazides
Pyrene hydrazide
BDP FL hydrazide
Cyanine3 hydrazide
Cyanine5 Boc-hydrazide
Cyanine5 hydrazide
Cyanine5.5 hydrazide
Cyanine7 hydrazide
Cyanine7.5 hydrazide
马来酰亚胺 Dye maleimides
BDP FL maleimide
Cyanine3 maleimide
Cyanine5 maleimide
Cyanine5.5 maleimide
Cyanine7 maleimide
Cyanine7.5 maleimide
Sulfo-Cyanine3 maleimide
Sulfo-Cyanine5 maleimide
羧酸 Carboxylic acids
Pyrenebutyric acid
BDP FL carboxylic acid
Cyanine3 carboxylic acid
Cyanine3.5 carboxylic acid
Cyanine5 carboxylic acid
Cyanine5.5 carboxylic acid
Cyanine7 carboxylic acid
Cyanine7.5 carboxylic acid
Sulfo-Cyanine3 carboxylic acid
Sulfo-Cyanine5 carboxylic acid
Sulfo-Cyanine7 carboxylic acid
点击化学试剂
非荧光炔烃 Non-fluorescent alkynes
Pentynoic acid STP ester
Biotin alkyne
非荧光叠氮化物 Non-fluorescent azides
Azidobutyric acid NHS ester
Biotin azide
寡核苷酸分析
亚磷酰胺 Phosphoramidites
FAM Phosphoramidite, 6-Isomer
Alkyne Amidite, hydroxyprolinol
Alkyne Phosphoramidite, 5'-terminal
DMS(O)MT aminolink C6
Long trebler phosphoramidite
Pyrene phosphoramidite dU
PCR/qPCR PCR/qPCR
ROX reference dye for qPCR
SYBR Green I Gel Staining Solution, 10000x
SYBR Green I for Real Time PCR, 100x
CPG固相修饰 CPG solid supports
Alkyne CPG modifier 500
TFA-Amino modifier CPG 500
三磷酸酯修饰 Modified triphosphates
Ami1-ddUTP
Ami1-dUTP